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Technologies > CloneBeacon™ / HiXP™
CloneBeacon™ / HiXP™Accelerated Protein Generation & Producer-Cell Discovery TechnologiesProprietary productivity-enrichment workflows designed to accelerate protein generation, reduce clone-screening burden, and support earlier identification of productive CHO cell populations. Designed to support pharma, CRO/CDMO, and biologics-development teams by shifting early CHO workflows from survival selection to productivity discovery. Available through: Strategic collaborations, sponsored evaluations, workflow-integration partnerships, and licensing discussions. Provisional patent applications filed. Technology OverviewTraditional CHO cell-line development can require prolonged selection, broad clone expansion, and expensive product-specific assays before high producers are identified. CloneBeacon™ / HiXP™ is designed to identify and enrich productive cell populations earlier using a linked small secreted reporter signal — small SSR™ — allowing productive wells or populations to be ranked before costly downstream expansion.
Core concept: Measure productivity earlier, advance only the strongest producers, and integrate the workflow into existing CHO development infrastructure.
Technology Proof Points1. From Survival Selection to Productivity DiscoveryCloneBeacon™ changes early CHO cell-line development from selection based mainly on cell survival to early ranking based on productivity-linked reporter output. 
Figure 1: CloneBeacon™ enables early, non-destructive productivity ranking before expensive clone expansion and product-specific assays. Core principle: replace late survival-based screening with early productivity-linked screening.
2. Two Complementary Workflow PathsHiXP™ and CloneBeacon™ provide two related entry points into biologics workflows.  
Figure 2: HiXP™ creates rapid protein-generation value; CloneBeacon™ creates producer-cell discovery value. The platform is differentiated by the combination — not any single component.
3. Iterative Productivity EnrichmentSmall SSR™-based screening enables non-destructive ranking so productive expression states can be enriched, expanded, and re-ranked. 
Figure 3: Transfect → rank by small SSR™ → enrich winners → re-expand → generate higher-productivity populations. Why it matters: the customer gets rapid evaluation protein now, while the same productivity signal guides selection of future producer candidates.
4. Early Detection of Productive Clonal Wells & Workflow IntegrationCloneBeacon™ supports detection of productive monoclonal and oligoclonal wells using a secreted productivity-linked reporter signal. It is designed to integrate into existing pharma, CRO, or CDMO workflows rather than replace established manufacturing infrastructure.  
Figure 4 & 5: Representative detection of productive monoclonal and oligoclonal CHO wells. Left Panel: 1 cell/well, Right Panel: 10 cells/well in suspension CHO cells via IVIS imaging following limiting dilution. Clonally derived high expressers can be identified just 8 days after transduction. Targeting Systems supplies expression constructs, small SSR™ detection reagents, and workflow support.
Potential Applications
Selected Commercial PositioningCloneBeacon™ / HiXP™ is designed as a deployable workflow platform with multiple partnership routes including:
Our initial goal is to identify evaluation partners interested in testing a construct plus small SSR™ detection workflow within existing CHO-development processes. Our TeamLean core team supported by domain experts in vector design, CHO cell-line development, biologics production, detection systems, and business development.
Prior R&D Support & Future DirectionPlatform development has been supported in part through prior NIH- and NSF-supported R&D programs. Targeting Systems is actively pursuing strategic collaborations in biologics expression, productivity-enrichment technologies, and accelerated producer-cell discovery workflows. Future development is focused on:
Interested in Strategic Collaborations or Evaluation Partnerships?Interested in learning more about CloneBeacon™ / HiXP™ productivity-enrichment technologies? |
